Schematic view of L3/p23 protease-inactivated Ad2 and Ad2-ts1 lacking functional protease. If the cysteine protease L3/p23 is chemically inactivated in isolated virus, Ad2 progresses to the nuclear pore complex, but fails to dissociate the capsid. The mutant Ad2-ts1, in turn, not only lacks functional protease but also contains an immature, nonproteolyzed capsid. Ad2-ts1 is defective at the endosome escape step. Modified from (1).
(1). Greber, U. F., et al. (1996) EMBO J. 15, 1766-1777.