|
|
Movies from
Suomalainen et al., J. Cell Biol. 144 (4): 657-672
|
view the abstract on pubmed |
click on an icon to see the corresponding movie |
|
Fig. 5. |
Time lapse fluorescence microscopy of wt Ad2 labeled with Texas red demonstrating that fast minus end-directed transport dominates over plus end-directed transport in TC7/MAP4/MTB-GFP cells. 104 frames of TR-Ad2 were recorded with intervals of 1.3 s starting 30 min p.i. (time stamp displaying minutes:seconds.centi-seconds). A GFP image is shown in the beginning. Bar = 10 µm |
|
|
Fig. 6. |
Fast minus and plus end-directed transport of endosomal ts1 virus labeled with Texas red. Virus was added to TC7/MAP4/MTB-GFP cells and 72 TR-images were recorded with intervals of 1.5 s starting 20 min p.i.. The GFP signal is indicated at the beginning. Bar = 10 µm. |
|
|
Fig. 7. Rapid switching between minus- and plus end-directed motions of wt Ad2 (bars = 5 µm) |
|
A: No drug-treated TC7/MAP4/MTB-GFP cells (64 frames at 1.2 s intervals). |
|
B: Taxol-treated cells (64 frames at 2.6 s intervals). |
|
C: Cells re-establishing their MTs after nocodazole wash out (41 frames at 1.8 s intervals). |
|
|
Fig. 9. Nuclear transport of wt Ad2 in control HeLa cells ( panel Aa , 43 frames at 2.6 s intervals) and periphery-directed transport in HeLa cells overexpressing dynamitin ( panel Ab , 37 frames at 2.1 s intervals). Nondirectional movements of Ad2 in nocodazole-treated HeLa cells ( panel Ac , 246 frames at 1.5 s intervals) and of virus at the cell surface ( panel Ad , 97 frames at 2.6 s intervals). Bar equals 5 µm. |
|
Fig. 9Aa |
|
Fig. 9Ab |
|
Fig. 9Ac |
|
Fig. 9Ad |